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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Inhibition of cyclin E1 sensitizes hepatocellular carcinoma cells to regorafenib by mcl-1 suppression

Fig. 2

CCNE1 expression suppressed sorafenib and regorafenib induced cell death. a The cell cycle of Huh7 cells transfected with the control or CCNE1 plasmid. b The cell viability of Huh7 cells transfected with the control or CCNE1 plasmid. c The cell viability of Huh7 cells transfected with the control or CCNE1 plasmid and treated with different concentrations of regorafenib or sorafenib. d Hoechst 33258 staining for apoptosis of Huh7 cells transfected with the control or CCNE1 plasmid and treated with 8 μM regorafenib or 5 μM sorafenib. f Annexin V/PI staining for apoptosis of Huh7 cells transfected with the control or CCNE1 plasmid and treated with 8 μM regorafenib or 5 μM sorafenib. f Western blot of CCNE1 and cleaved caspase 3/8 in Huh7 cells transfected with the control or CCNE1 plasmid and treated with 8 μM regorafenib or 5 μM sorafenib. The western blots and flow cytometry were repeated for 3 times, and representative data were shown. N = 3 for b-e. *, P < 0.05, **, P < 0.05

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