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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Characterization of CD44 intracellular domain interaction with RUNX2 in PC3 human prostate cancer cells

Fig. 2

Characterizing the expression of RUNX2 in PCa cell lines. a. The real-time PCR analysis of RUNX2 expression in PC3 (lane 1), LNCaP (lane 2), and PCa2b (lane 3) cells. b. Immunoblotting analysis with an antibody to Runx2 (top panel) and GAPDH (bottom panel). Equal amounts of protein lysates (40 μg) made from PC3 (lane 1), LNCaP (lane 2), PCa2b (lane 3). c. Equal amounts of PC3 and PC3/AR+ were immunoblotted with RUNX2 antibody. GAPDH was used as a loading control for real-time PCR and immunoblotting analysis (a-c). The results represent one of three separate experiments performed with the same results

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