Table 1 Sequences of the primers
From: miR-449a regulates insulin signalling by targeting the Notch ligand, Jag1 in skeletal muscle cells
Gene name | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
|---|---|---|
Jag1 | TGCCTCTGTGAGACCAACTG | AGGGGTCAGAGAGACAAGCA |
Hes1 | GTCTACACCAGCAACAGTGG | CGTCAGAAGAGAGAGGTGGG |
Hey1 | TCAGAGCAGTGAGGTGAAGG | AGTGCAGGCAAGGTCTACAT |
Jag1 3’UTR | AATTAATTGTGTGAAGTTGGAAGCA | TGTTCCATGTTTTCATACAAAAAATT |
Mutant 3’UTR | ACTTTGATTTCCTCACTTAAGGCAGGTCAGGTACTCTACGGCAAATCTAAACAGTG | CACTGTTTAGATTTGCCGTAGAGTACCTGACCTGCCTTAAGTGAGGAAATCAAAGT |