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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: Histone deacetylase 1 induced by neddylation inhibition contributes to drug resistance in acute myelogenous leukemia

Fig. 7

Nedd8 facilitated ubiquitination of HDAC1. a HL-60 cells transfected with constructs encoding HDAC1, His-ubiquitin (His-Ub), Flag-NUB1 (NEDD8 ultimate buster 1), and Myc-Mdm2 following incubation with MG132, a protease inhibitor. Ni2+ pull-down assay and Western blot analysis of (Ub)n-HDAC1 and Flag-NUB1 expression. b The Ni2+ pull down assay was evaluated by Western blot with appropriate antibodies in HL-60 cells expressing HDAC1, Flag-NUB1, His-ubiquitin, Myc-Mdm2, and NEDP1

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