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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Histone deacetylase 1 induced by neddylation inhibition contributes to drug resistance in acute myelogenous leukemia

Fig. 6

Neddylation of HDAC1. a Western blot analysis of Nedd8 substrates isolated by His purification in HL-60 cells co-transfected with Flag-HDAC1 and different dose of His-Nedd8. b Western blot analysis of Nedd8-HDAC1 (65kD) in the proteins purified by NTA-Ni2+ agarose beads from the HL-60 cells stably expressing His-Nedd8 by using primary antibody for HDAC1. HDAC1 (65kD) in HL-60 cells without His-Nedd8 transfection was also analyzed. c Western blot analysis of Nedd8 substrates of HL-60 cells expressing Flag-HDAC1 and His-Nedd8 after 16 h of treatment with MLN4924, an inhibitor of Nedd8-activating enzyme. d Western blot analysis of HDAC1 expression in HL-60 and K562 cells after MLN4924 treatment. e Immunoprecipitation assay of the interaction of HDAC1 and Nedd8 in HL-60 and K562 cells

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