Fig. 6

Silencing of SAE1 expression inhibits glioma development in vivo. a A schematic diagram was recorded the dates of treatments in mice. b The tumors isolated from xenografted nude mice, which were treated with siSAE1, were much smaller than the control group treated with siCon. After the tumor growth for 12 days (cell injection for 26 days), nude mice were killed to isolate tumor. c Tumor growth curves of xenograft nude mice. Data were presented as mean ± SD, *p < 0.05 is comparing with the control group. d The average weight of the dissected tumors (on the 29th day) was much smaller than the control group. Data were presented as mean ± SD. e The expression of SAE1, AKT signaling pathway proteins, typical cell cycle-related proteins (CDK2, Cyclin D1, p21) and typical apoptosis-related proteins (Bcl-2, active Caspase-3) in xenografted nude mice tumors. siSAE1: nude mice inoculated with U87 cells which were treated with SAE1 siRNA by intratumoral injection. siCon: nude mice inoculated with U87 cells which were treated with non-targeting siRNA by intratumoral injection. S-AKT: the SUMOylated AKT