Fig. 5

SAE1 promotes glioma development in vivo. a A schematic diagram was recorded the dates of treatments in SAE1-overexpressing mice. b The tumors isolated from xenograft nude mice, which were injected with pFlag-SAE1-overexpressing U87 cells, were much bigger than the control group injected with pFlag-containing U87 cells. After the tumor growth for 21 days (cell injection for 35 days), nude mice were killed to isolate tumor. c Tumor growth curves of xenograft nude mice. Data were presented as mean ± SD, *p < 0.05, **p < 0.01. d The average weight of the dissected tumors (on the 35th day) was much heavier than the control group. Data were presented as mean ± SD, *p < 0.05, **p < 0.01. e The expression of SAE1, AKT signaling pathway proteins, typical cell cycle-related proteins (CDK2, Cyclin D1, p21) and typical apoptosis-related proteins (Bcl-2, active Caspase-3) in random chosen nude mouse xenografted tumors. SAE1: nude mice inoculated with pFlag-SAE1-overexpressing U87 cells. Con: the control group, nude mice inoculated with pFlag-containing U87 cells. S-AKT: the SUMOylated AKT protein