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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: SAE1 promotes human glioma progression through activating AKT SUMOylation-mediated signaling pathways

Fig. 3

SAE1 protein enhances Akt SUMOlyation and phosphorylation. a The expression of SAE1, SUMO1, p-AKT in 3 random chosen HGTs and their paired PBTs. b The H&E staining and the immunohistochemical staining of SAE1, SUMO1, p-AKT in 2 cases of HGTs with a high pathologic grade and their paired PBTs. c The expression of SAE1, SUMOylated Akt, p-Akt (Ser473) and global SUMOylation were measured in 2 cases of HGTs with a high pathologic grade and their paired PBTs. HGTs: human glioma tissues; PBTs: para-cancerous brain tissues; case #4–6: glioma tissues respectively with the pathologic grade IV, III and I. d The levels of SAE1 and global SUMO1 modification were detected in U87 and U251 cells. (e-f) SAE1 activates Akt SUMOylation and phosphorylation (Ser473) in glioma cells. After the plasmid SAE1 or siSAE1 were transfected into U87 or U251 cells for 48 h, cell pellets were collected to extract cellular lysates to enrich SUMOylated proteins by IP, from which the protein were eluted to detect SUMO1 modified proteins and SUMOylated AKT. Cell lysates were also detected by Western blotting respectively with anti-SUMO1, anti-SAE1, anti-AKT, anti-p-AKT and β-actin antibodies. S-AKT: the SUMOylated AKT protein. p-AKT: phosphorylated Akt. Con: pFlag empty vector as a mock control. SAE1: pFlag-SAE1. siCon: non-targeting control siRNA. siSAE1: The SAE1-specific siRNA3. IP: immunoprecipitation, IB: immunoblot, Input: same account of cell lysate to load

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