Fig. 2

Effect of NR6A1 silencing on lipid content in HepG2 cells. HepG2 cells were transfected with scrambled or NR6A1 siRNA for 24 h and then incubated under normal growth medium or medium containing OA (50 μM) or PA (100 μM) for another 24 h. (a) Cells were fixed and stained by Oil Red O and then observed under fluorescence microscopy. Representative images were shown. Bar = 100 μm. (b) Quantification by extracting Oil Red O stained lipid droplets with 100% isopropanol and OD (510 nm) was measured. n = 3. **P < 0.01., *P < 0.05. HepG2 cells were transfected with scrambled or NR6A1 siRNA for 48 h. The mRNA levels of lipogenic genes (c), PEPCKc (d) and CPT1a (e) were determined by using qPCR method. Data are obtained from 3 independent experiments. **P < 0.01., *P < 0.05. (f & g)The animals were starved overnight for 16 h. After that, NR6A1 expression in liver was quantified by using qPCR method. (f) C57BL/6 vs. db/db mice (n = 4). (d) Wistar vs. GK rats (n = 4). (h) The expressions of cancer stem cell markers in control and NR6A1 silenced cells. n = 3