Fig. 1
From: ErbB4 promotes malignant peripheral nerve sheath tumor pathogenesis via Ras-independent mechanisms
ErbB4 is commonly expressed in human MPNSTs and MPNST-derived cell lines. a-f Representative images of erbB4 immunostaining of FFPE sections of human MPNSTs demonstrates different grades of erbB4 staining compared to isotype matched negative control b. The erbB4 grading is represented numerically on a scale between 1+ to 4+. A subset of erbB4 positive tumor samples displayed prominent nuclear staining (c-f) and others displayed exclusive non-nuclear membranous staining (a). Red channel (Alexa 568, erbB4). Blue channel (Hoechst, nuclei). Scale bar represents 100um. g Real time PCR analysis showing relative mRNA expression of erbB4 mRNA using non-isoform discriminatory PCR primers; mRNA levels are shown relative to human Schwann cells. GAPDH mRNA was used as the loading control for normalization. h ErbB4 protein expression of by immunoblot analysis of whole cell lysates derived from human MPNST derived cell lines. GAPDH was used as a loading control