Skip to main content

Advertisement

Fig. 7 | Cell Communication and Signaling

Fig. 7

From: TNF-α promotes human antibody-mediated complement-dependent cytotoxicity of porcine endothelial cells through downregulating P38-mediated Occludin expression

Fig. 7

SB203580 or loss of P38 suppressed the decrease of Occludin expression in PIECs exposed to TNF-α. a PIECs were pretreated with DMSO, or SB203580 for 30 min and then exposed to rhTNF-α (20 ng/ml) for 0, 12, or 24 h. Total RNA was collected and the mRNA level of Occludin was measured by RT-PCR. b PIECs were pretreated with DMSO, or SB203580 for 30 min and then exposed to rhTNF-α (20 ng/ml) for 0, or 24 h. Lysates were analyzed by immunoblotting with antibodies against Occludin and actin. c The siP38 oligos and control siRNA oligo were transfected into PIECs. After 48 h, the transfected cells were treated with or without rhTNF-α (20 ng/ml) for 24 h. Total RNA was collected and the mRNA level of Occludin was measured by RT-PCR. d The siP38 oligos and control siRNA oligo were transfected into PIECs. After 48 h, the transfected cells were treated with rhTNF-α (20 ng/ml) for 0, or 24 h. Lysates were analyzed by immunoblotting with antibodies against Occludin and actin. Data are representative of at least three independent experiments (mean ± SEM). **p < 0.01, ***p < 0.001 by Student’s t test

Back to article page