Fig. 6

In a human antibody-mediated CDC model, SB203580 or loss of P38 suppressed the cytotoxicity of PIECs exposed to TNF-α. a PIECs were treated with rhTNF-α (20 ng/ml) for 0, 15, 30, 60 min. Lysates were analyzed by western blotting with antibodies against pJNK, JNK, pERK, ERK, pP38, P38 and actin. b-d PIECs were pretreated with DMSO, SP600125 (b), PD98059 (c) or SB203580 (d) for 30 min and treated with rhTNF-α (20 ng/ml) for 24 h or 48 h and then exposed to 10% or 20% human serum (HS) to induce antibody-mediated CDC. e The siP38 oligos and control oligo were transfected into PIECs. After 48 h, the transfected cells were treated with rhTNF-α (20 ng/ml) for 48 h and then exposed to 10% or 20% HS to induce antibody-mediated CDC. Data are representative of at least three independent experiments (mean ± SEM). *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t test. N.S. = no significance