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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: TNF-α promotes human antibody-mediated complement-dependent cytotoxicity of porcine endothelial cells through downregulating P38-mediated Occludin expression

Fig. 2

Human TNF-α ectopically expressed in PIECs (using an adenoviral system) augmented the cytotoxicity of PIECs in a human antibody-mediated CDC model. a The plasmid which expressed the human TNF-α gene was constructed using an adenovirus (Adv) system. The Adv was packaged. PIECs were infected with Adv-TNF-α, or Adv-EV (empty vector) for 72 h. Total RNA was collected for RT-PCR analysis. The mRNA level was assessed with a human TNF-α primer, and normalized to pig GAPDH. b PIECs were infected with Adv-TNF-α, or Adv-EV for 72 h. The supernatant was collected for enzyme-linked immunosorbent assay (ELISA). c PIECs were infected with Adv-TNF-α, or Adv-EV for 72 h, and then exposed to human serum to induce antibody-mediated CDC. Data are representative of at least three independent experiments (mean ± SEM). ***p < 0.001 by Student’s t test. N.D. = not determined

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