Minocycline impairs TNF-α-induced cell fusion of M13SV1-Cre cells with MDA-MB-435-pFDR1 cells by suppressing NF-κB transcriptional activity and its induction of target-gene expression of fusion-relevant factors

Table 1 Antibodies used in this study

Antibody	Source	Clone	Company
anti-NF-κB p65	rabbit monoclonal	D14E12	Cell Signaling^a
anti-phospho-NF-κB p65(S536)	rabbit monoclonal	93H1	Cell Signaling^a
anti-SRC	rabbit monoclonal	36D10	Cell Signaling^a
anti-phospho-SRC (Tyr416)	rabbit polyclonal		Cell Signaling^a
anti-IKK-α	rabbit polyclonal		Cell Signaling^a
anti-IKK-β	rabbit monoclonal	2C8	Cell Signaling^a
anti-phospho-IKK-α/β (S176/180)	rabbit monoclonal	16A6	Cell Signaling^a
anti-IκBα	rabbit monoclonal	44D4	Cell Signaling^a
anti-phospho-IκBα (S32)	rabbit monoclonal	14D4	Cell Signaling^a
anti-SAPK/JNK	rabbit polyclonal		Cell Signaling^a
anti-phospho-SAPK/JNK(T183/Y185)	mouse monoclonal	G9	Cell Signaling^a
anti-p38 MAPK	rabbit polyclonal		Cell Signaling^a
anti-phospho-p38 MAPK (T180/Y182)	rabbit monoclonal	3D7	Cell Signaling^a
anti-p44/42 MAPK (Erk1/2)	rabbit polyclonal		Cell Signaling^a
anti-phospho-p44/42 MAPK (T202/Y204)	rabbit polyclonal		Cell Signaling^a
anti-TNFR1	rabbit monoclonal	C25C1	Cell Signaling^a
anti-TRAF2	rabbit monoclonal	C192	Cell Signaling^a
anti-eIF4E	rabbit monoclonal	46H6	Cell Signaling^a
anti-β-actin	mouse monoclonal	AC-74	Sigma-Aldrich^b
ALEXA488-conjugated goat-anti-rabbit IgG (H + L)	rabbit polyclonal		ThermoFisher Scientific GmbH^d
anti-mouse-IgG-HRP	mouse polyclonal		Cell Signaling^a
anti-rabbit-IgG-HRP	rabbit polyclonal		Cell Signaling^a
anti-rabbit IgG light-chain specific-HRP	mouse monoclonal	D4W3E	Cell Signaling^a
IgG	rabbit polyclonal		Merck^c

^a Cell Signaling Technology Europe B.V., Frankfurt am Main, Germany; ^b Sigma-Aldrich, Taufkirchen, Germany; ^c Merck, Darmstadt, Germany; ^d ThermoFisher Scientific GmbH, Schwerte, Germany

ISSN: 1478-811X