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Fig. 8 | Cell Communication and Signaling

Fig. 8

From: Minocycline impairs TNF-α-induced cell fusion of M13SV1-Cre cells with MDA-MB-435-pFDR1 cells by suppressing NF-κB transcriptional activity and its induction of target-gene expression of fusion-relevant factors

Fig. 8

Minocycline suppresses TNF-α-induced NF-κB-dependent expression of MMP9 and ICAM1. NF-κB-binding activity on the promoter regions of ICAM1 and MMP9 was analysed by ChIP assay. a Schematic overview of the PCR and qPCR amplification strategy of MMP9 and ICAM1 promotor regions. b PCR data. c Validation of PCR data by qPCR. Representative data and the means ± STD of at least two independent experiments for MDA-MB-435-pFDR1 cells and three independent experiments for M13SV1-Cre cells are shown. Statistical analysis was conducted with the Kruskal-Wallis test: * = p < 0.05; ** = p < 0.01; *** = p < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X