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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Minocycline impairs TNF-α-induced cell fusion of M13SV1-Cre cells with MDA-MB-435-pFDR1 cells by suppressing NF-κB transcriptional activity and its induction of target-gene expression of fusion-relevant factors

Fig. 2

Minocycline impairs the TNF-α-induced activation of SRC kinase and of MAPKs. M13SV1-Cre cells and MDA-MB-435-pFDR1 cells were treated with or without TNF-α (100 ng/ml) and with or without minocycline (20 μM) for 72 h. Whole cell lysates were collected, and constitutive and phosphorylated protein levels were analysed by western blotting: a SRC/ phos-SRC, b ERK1/2/ phos-ERK1/2, c p38/ phos-p38, and d JNK/ phos-JNK. Relative native and phosphorylated protein levels were normalised to the level of housekeeping gene expression and in relation to the control, which was set to 1. Representative data from at least three independent experiments are shown. Statistical analysis: ANOVA F-test and Scheffé post hoc test: * = p < 0.05; ** = p < 0.01; *** = p < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X