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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Minocycline impairs TNF-α-induced cell fusion of M13SV1-Cre cells with MDA-MB-435-pFDR1 cells by suppressing NF-κB transcriptional activity and its induction of target-gene expression of fusion-relevant factors

Fig. 1

Minocycline inhibits TNF-α-induced TRAF2-TNFR1 receptor binding. M13SV1-Cre cells and MDA-MB-435-pFDR1 cells were pre-treated with 100 μM minocycline for 24 h. Cells were additionally stimulated with 100 ng/ml TNF-α either for 2 h for western blot analysis (a) or for 1 h for co-immunoprecipitation studies (b). Co-immunoprecipitation was either performed using a TNFR1 antibody or a normal anti-rabbit IgG antibody. Western blotting was carried out by using the TRAF2 or the SRC antibody. Representative data from at least three independent experiments are shown

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