Fig. 7
From: YAP promotes neural crest emigration through interactions with BMP and Wnt activities
YAP is necessary for activity of the canonical Wnt pathway. Transverse sections of embryos co-electroporated with the 12TOPFLASH-d2EGFP reporter of canonical Wnt activity (green) and either a control scrambled RNA (a-a”), or shYAP (b-b″, red). Note the presence of double positive cells in controls (arrowheads in a’ and a” showing premigratory NC and arrows pointing to emigrating progenitors). In contrast, most shYAP+ progenitors are BRE-negative in the dorsal NT of shYAP-treated embryos (short arrows in b′,b″). (c) Left and right graphs show quantification of the percentage of double labeled progenitors out of total RFP+ cells in premigratory (*p < 0.001) and emigrating (**p < 0.0004) NC cells, respectively. (d) Left and right graphs show quantification of the percentage of double labeled progenitors out of total RFP+ cells in premigratory and emigrating NC cells, respectively. (*p < 0.03, **p < 0.0001, ***p < 0.00001). Bar = 50 μM