Fig. 1
From: YAP promotes neural crest emigration through interactions with BMP and Wnt activities
Expression of YAP-immunoreactive protein in premigratory and emigrating NC of avian embryos. Transverse sections at (a,a’) early epithelial somite level showing YAP expression in cytoplasm and nuclei (arrowheads) of cells at the premigratory domain of the dorsal neural tube (NT). Ectoderm (Ec) and notochord (No) are also positive. (b-b″) Dissociated somite level with migratory NC. Note in b,b′ co-expression of YAP (green) and HNK-1 (red) in emigrating progenitors adjacent to the NT (arrowheads in b′). In contrast, cells migrating ventrally through sclerotome are HNK-1+/YAP-negative (b″, arrows). The dorsal NT remains positive and also the dermomyotome (DM). (c-c″) Midbrain level showing positive YAP signal in dorsal NT, YAP+/HNK-1+ NC cells between NT and ectodem (arrowheads in c′), but HNK-1+/YAP-negative in nascent ganglion (arrows in c″). Nuclei were stained with Hoechst. Bar = (a-c), 50 μM