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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: A p53/TIAF1/WWOX triad exerts cancer suppression but may cause brain protein aggregation due to p53/WWOX functional antagonism

Fig. 2

WWOX inhibits cell proliferation and migration. a MEF cells were seeded on a 24-well plate and cultured for indicated periods. Live cell numbers were counted using a hemocytometer. b Wwox knockout MEF cells had a higher proliferation rate than wild type cells during culturing for 48 h (Mean ± S.D., n = 3, Student’s t test, p < 0.05). c Wwox−/− and Wwox+/− MEF cells migrated significantly faster than wild type Wwox+/+ cells (n = 3, Student’s t test). d Wild type cells migrated collectively, while WWOX-deficient cells migrated individually (see Additional file 2: Video S1 and Additional file 3: Video S2). e, f Breast MDA-MB-231 cancer cells were transfected with the indicated constructs by electroporation. Transiently overexpressed WWOX suppressed cell migration, whereas dnWWOX and WWOX siRNA constructs enhanced the migration. Dn: dominant negative; WWOXww: WW domain of WWOX; Scram: scramble control; WWOXsi#1 and #2: siRNAs targeting human/murine WWOX (n = 3, Student’s t test) [47]. g, h During treatment for 48 h, TGF-β1 promoted wild type cell migration, but suppressed Wwox knockout cell migration in a dose-dependent manner (n = 3, Student’s t test). i. Both TGF-β1 and TGF-β2 promoted wild type MEF cell migration during treatment for 48 h. TGF-β2 is more effective in suppressing Wwox knockout cell migration than TGF-β1 (n = 3, Student’s t test)

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