Fig. 1

IL-6 induces the expression of two different isoforms of SOCS3. HepG2, HeLa, and HEK293 cells were stimulated with hyIL-6 (20 ng/mL) for 90 min or left untreated as indicated. SOCS3 and tubulin protein expression were evaluated by Western blotting. Arrowheads indicate the long and short isoforms of SOCS3. Representative results of n = 3 independent experiments are shown