Fig. 7

Coincidently, MEKi reduces expression of IFNB1 gene repressor, PRDI-BF1, by dampening STAT3 activation but this is an RSVA2 specific effect. a qRT-PCR analysis of PRDI-BF1 mRNA in AECs pretreated with DMSO, MEKi, or anti-IFN-β antibody for 1h followed by infection with or without RV2 or RSVA2 or UV-inactivated viruses for 24h (MOI 0.1). For the poly(I:C) model (right panel), qRT-PCR analysis of PRDI-BF1 mRNA was assessed in AECs pretreated with DMSO or MEKi for 1h and subsequently stimulated with or without poly(I:C) for 1-24h. b Immunoblot analysis (with anti-pSTAT3s, anti-STAT3 and anti-α-tubulin) of AECs pretreated with DMSO, MEKi, or anti-IFN-β antibody for 1h followed by infection with or without RV2 or RSVA2 or UV-inactivated viruses for 24h (MOI 0.1). For the poly(I:C) model (right panel), immunoblot analysis of AECs pretreated with DMSO or MEKi for 1h and subsequently stimulated with or without poly(I:C) for 1-24h. Each symbol () represents a donor and the horizontal bars represent the grand median in a (n = 6). Data are presented as median ± interquartile range in a (poly(I:C) model, n = 6). b is a representative immunoblot (n = 6). Statistical analysis was performed with the Wilcoxon signed-rank test. * p < 0.05 indicates statistical significance