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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: MEK inhibition drives anti-viral defence in RV but not RSV challenged human airway epithelial cells through AKT/p70S6K/4E-BP1 signalling

Fig. 6

MEKi promotes deactivation (phosphorylation) of translational repressor 4E-BP1 but activates p70S6K. a Immunoblot analysis (with anti-4E-BP1 and anti-GAPDH) of AECs pretreated with DMSO, MEKi, or anti-IFN-β antibody for 1h followed by infection with or without RV2 or RSVA2 or UV-inactivated viruses for 24h (MOI 0.1). For the poly(I:C) model (right panel), immunoblot analysis of AECs pretreated with DMSO or MEKi for 1h and subsequently stimulated with or without poly(I:C) for 1-4h. Arrows show the α, β, and γ forms of 4E-BP1. α, β, and γ in order of increasing electrophoretic mobility, where γ form is the hyperphosphorylated form of 4E-BP1. β is phosphorylated to a lesser extent that γ, and α is the hypophosphorylated form. b Immunoblot analysis (with anti-peIF4E, anti-eIF4E and anti-GAPDH) of AECs pretreated with DMSO, MEKi, or anti-IFN-β antibody for 1h followed by infection with or without RV2 or RSVA2 or UV-inactivated viruses for 24h (MOI 0.1). For the poly(I:C) model (right panel), immunoblot analysis of AECs pretreated with DMSO or MEKi for 1h and subsequently stimulated with or without poly(I:C) for 1-4h. c Immunoblot analysis (with anti-pp70S6K, anti-p70S6K, and anti-GAPDH) of AECs pretreated DMSO, MEKi, or anti-IFN-β antibody for 1h followed by infection with or without RV2 or RSVA2 or UV-inactivated viruses for 24h (MOI 0.1). For the poly(I:C) model (right panel), immunoblot analysis of AECs pretreated with DMSO or MEKi for 1h and subsequently stimulated with or without poly(I:C) for 1-4h. d qRT-PCR analysis of ISG15 mRNA in AECs pretreated with DMSO, MEKi, or anti-IFN-β antibody for 1h followed by infection with or without RV2 or RSVA2 or UV-inactivated viruses for 24h (MOI 0.1). a, b, and c are representative immunoblots (n = 6). Each symbol () represents a donor and the horizontal bars represent the grand median in d (n = 6). Statistical analysis was performed with the Wilcoxon signed-rank test. * p < 0.05 indicates statistical significance

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