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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Targeting of the AXL receptor tyrosine kinase by small molecule inhibitor leads to AXL cell surface accumulation by impairing the ubiquitin-dependent receptor degradation

Fig. 2

The significant increase of AXL protein levels by 0.5 μM BMS777607 treatment is depending on culture conditions. Combination treatments of cycloheximide (CHX) and BMS777607 (BMS) were performed to discriminate the effects of BMS on translation and proteolysis of AXL. Additionally, we analyzed the impact of serum conditions (+/− FCS) and accumulation of AXL ligand GAS6 by medium exchange prior to the combinatorial CHX and BMS treatment. a AXL protein levels were increased significantly by BMS treatment in Hs578T cells. This effect is lost by exchange to serum containing medium. b AXL protein levels were increased significantly by BMS treatment independent of cell culture medium conditions or serum concentration in H292 cells. c H1792 cell responded to BMS treatment only after replacement of serum containing cell culture medium to serum depleted medium. d and e Representative western blots of biological replicates are shown. Mean values and SEM of four (H292) or five (Hs578T and H1792) independent experiments are shown. Differences with *P < .05, **P < .01, ***P < .001 and ****P < .0001

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