Fig. 6

CD93 colocalizes with the small GTPase Rab5. HUVECs were transfected or not with CD93-YFP, then they were detached from the plate, resuspended in complete growth medium, plated on the substrate, fixed at early (a) and late (b) degrees of cell spreading, and subjected to immunofluorescence analysis. a: Untransfected cells (CD93) were stained with phalloidin, anti-Rab5, and anti-CD93 antibodies. Transfected cells (CD93-YFP) were stained with phalloidin and anti-Rab5 antibodies. Scale bars, 7 μm. b: Untransfected cells (CD93) were stained with anti-CD93 and anti-Rab5 antibodies. Transfected cells (CD93-YFP) were stained for Rab5. A dotted line indicates nucleus boundary. Scale bars, 7 μm. In transfected cells, exogenous CD93 was imaged as green. Merged, DIC, and wdc images between CD93 and Rab5 are shown. For each condition, Manders and Costes quantitative analyses of Rab5 colocalization with CD93 and vice versa are reported (n = 5–8 cells)