Fig. 4

CD93 colocalizes with MMRN2 in endocytic vesicles. The CD93-YFP construct was transfected or not into HUVECs. Cells were detached from the plate, resuspended in complete growth medium, plated, fixed at different times after plating, and analyzed by confocal microscopy. a, b: Untransfected ECs at early and late phases of spreading were stained using phalloidin, anti-CD93, and anti-MMRN2 antibodies. Merged, DIC, and wdc images between CD93 and MMRN2 are shown. Scale bars, 7 μm. In b, the wdc picture shows a high magnification of the CD93^+ve vesicles displayed into the dashed square. Scale bar, 4 μm. c, d: Transfected early and late spreading cells were stained for MMRN2. Exogenous CD93 was imaged as green. Overlay of stained cells and wdc images are shown. Scale bars, 7 μm. For each condition, Manders and Costes quantitative analyses of MMRN2 colocalization with CD93 and vice versa are reported (n = 5–7 cells)