Fig. 1From: The small GTPase Rab5c is a key regulator of trafficking of the CD93/Multimerin-2/β1 integrin complex in endothelial cell adhesion and migrationDuring the initial phases of spreading, CD93 is sorted towards the actin-rich apical bud. HUVECs were pretreated or not for 30 min with latrunculin B, then they were detached from the plate, resuspended in complete growth medium, and plated on the substrate in the presence or not of latrunculin B. 5 min after plating, cells were fixed and analyzed by immunofluorescence using phalloidin, anti-Moesin, and anti-CD93 antibodies. The apical bud is delimited by a dotted line. Arrowheads indicate small vesicles beneath the apical bud. Differential interference contrast (DIC) images of stained cells are shown. Scale bars represent 5 μmBack to article page