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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Interleukin-32θ inhibits tumor-promoting effects of macrophage-secreted CCL18 in breast cancer

Fig. 4

Blocking CCL18 signaling reduces PKCδ-mediated signaling and metastasis-related factors. MDA-MB-231-EV cells were treated with CM in the absence or presence of CCL18 neutralizing antibodies. IgG antibody was used as a negative control. a mRNA expression levels of pro-malignancy factors were determined by real-time PCR (n = 5). b COX-2, E-cadherin, phosphor-STAT3 (Y705), and IκBα protein expression was analyzed by western blotting. c MMP-9 expression was detected by zymography. d Protein secretion of GM-CSF was measured by ELISA (n = 3). Western blot or zymography images are the representative results of three independent experiments. e The invasion or migration abilities of cells were performed using Matrigel-coated or non-coated transwell chambers. Related images were obtained from an upright microscope. f Migration and invasion intensities were quantitated based on an OD at 620 nm (n = 3). Scale bar, 100 μm (migration) and 200 μm (invasion). All data are presented as the mean ± SEM and are analyzed using the Student’s t-test: *, p < 0.05

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