Fig. 3
From: Interleukin-32θ inhibits tumor-promoting effects of macrophage-secreted CCL18 in breast cancer
IL-32θ interacts with PKCδ, and subsequently inhibits the NF-κB and STAT3 pathways in MDA-MB-231 cells. a IL-32θ interacts with PKCδ upon stimulation with PMA or CM. Immunoprecipitation was performed using anti-Myc antibody. b-c MDA-MB-231-EV and MDA-MB-231-IL-32θ cells in the absence or presence of CM of THP-1 macrophages were harvested and separated into cytosol and nuclear fractions. NF-κB nuclear translocation and phosphorylation of IκBα and STAT3 was detected by western blot. d-e Immunofluorescence assay to detect p65 (d) or STAT3 (e) (red) and Myc-IL-32θ (green) localization (scale bar, 5 μm). Nuclei were stained with DAPI (blue). Western blot or immunofluorescence images are the representative results of three independent experiments