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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: Tumor-secreted PAI-1 promotes breast cancer metastasis via the induction of adipocyte-derived collagen remodeling

Fig. 7

Clinical relevance of PAI-1 and PLOD2 in mammary tumor-adipose periphery. a Kaplan–Meier (KM) analysis of relapse-free survival of breast cancer patients from KM plotter (http://kmplot.com/analysis). PAI-1 in breast cancer patients (n = 3971), in HER2 negative breast cancer patients (n = 800), in ER negative breast carcinoma patients (n = 801), and in triple negative breast carcinoma patients (n = 255). b Masson trichrome stained of invasive breast tumors, sections from far away normal mammary adipose tissue, tumors center, and the invasive front of tumors, collagen fibrillar was stained in blue and the nucleus in red (original magnification × 100). c The degree of collagen fibrillar organization was evaluated by a pathologist. The score (from 0 to 6) indicates the degree of fibrillar organization. A higher score indicated greater fibrillar organization. The *P-values < 0.05 were considered statistically significant for all tests. d Western blot showing expression of PLOD2 in normal adipose tissue (N) and the corresponding peri-tumor adipose tissue (P) from nine invasive breast cancer patients. e The relative PLOD2 expression at protein levels was analyzed by using GAPDH as the loading control (n = 9). **P-values < 0.01 were considered statistically significant for all tests. f Histological examination of PAI-1 and PLOD2 was visualized (brown) in adipocytes located adjacent to invading cancer cells (Invasive front) and the tumor center. g Correlation analysis of PLOD2 in peritumoral adipose tissue and PAI-1 in breast cancer patients. h Schematic diagrams show the heterotypic interaction between breast cancer cells and adipocytes in the breast cancer microenvironment. Tumor-derived PAI-1 promotes adipocyte-derived collagen reorganization in a paracrine manner. PAI-1 secreted from breast cancer cells can promote PLOD2 expression in adipocytes via activating the PI3K/AKT-FOXP1 axis. Activation of PLOD2 induces the linear organization of adipocyte-derived collagen, which leads to tumor cells metastasis

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