Skip to main content
Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Androgen promotes differentiation of PLZF+ spermatogonia pool via indirect regulatory pattern

Fig. 3

Impact of androgen on SPCs differentiation in SPCs-Sertoli cells co-culture system. Morphology of total testicular cells isolated from 5-day mice was displayed after 12 h’ culture (a). The morphology of co-culture system after two passages was exhibited (b). More Aal spermatogonia (red arrows) were observed in DHT treat group (c) while more clusters (red arrow heads) formed in bicalutamide treat group (d). The ratio of spermatogonia cluster/chain was calculated after DHT or bicalutamide treatment in co-culture system, n = 5 (e). Expression levels of Plzf, Arc-kit, Gfrα1Tex14 and Id4 in co-culture system after DHT and/or bicalutamide treatment were evaluated by qRT-PCR, n = 4, and relative expression of indicated genes are quantitatively analyzed after normalization to Gapdh. 1, DHT; 2, bicalutamide; 3, DMSO (f). Impact on expression of AR and PLZF in co-culture system after DHT and bicalutamide treatment was determined by western blot, n = 6 (g), and the relative expression levels of AR and PLZF were statistically analyzed. 1, DHT+/bicalutamide-; 2, DHT-/bicalutamide-, 3, DHT+/bicalutamide+; 4, DHT-/bicalutamide+  (h). DHT aggravated the decline of PLZF expression caused by PLZF siRNA, while bicalutamide alleviated the knockdown effect, n = 5 (i), and the relative expression levels of PLZF were statistically analyzed, 1, DHT+/Plzf siRNA+; 2, DHT+/control siRNA+; 3, bicalutamide+/Plzf siRNA+; 4, bicalutamide+/control siRNA+. (j). Data represent means ± SD (*p < 0.05, **p < 0.01). Bar = 20 μM

Back to article page