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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Androgen promotes differentiation of PLZF+ spermatogonia pool via indirect regulatory pattern

Fig. 2

SPCs-somatic cells co-culture system was able to be maintained in vitro for short term. SPCs-somatic cells co-culture system stably expressed MVH ((a) bright filed, (b) MVH, (c) dapi, (d) merge), PLZF ((e) bright filed, (f) PLZF, (g) dapi, (h) merge), CD9 (i) bright filed, (j) WT1, (k) dapi, (l) merge), AR ((m) bright filed, (n) AR, (o) dapi, (p) merge) and WT1 ((q) bright filed, (r) WT1, (s) dapi, (t) merge). RT-PCR (n = 2) results exhibit the co-culture system expresses somatic markers ((u) 1.Gapdh, 2.Sox9, 3. Sma, 4.Coup-tf II, 5. Wt1, 6.Mvh), Ar (V. 1) and germline markers (v) 2.Scp3, 3.ckit, 4.Integrin-αV, 5.Cd9, 6.Integrin-β1, 7. Integrin-a6, 8. Plzf, 9. Mvh) after 3 passages. Western blot result confirmed this co-culture system was sensitive to DHT or bicalutamide stimulation, 1, DMSO; 2, 10nM DHT; 3, 10nM bicalutamide, n = 3 (w)

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