Fig. 5

Ori exerted anti-inflammatory effects not by the regulation of antioxidative effects. After pretreatment of brusatol (300 nM) for 1 h, cells were exposed to Ori (10 μM) for 6 h and then treated with LPS (1 μg/ml) and ATP for 1 h and 40 min, respectively. a Protein expressions of NLRP3, CASPASE-1, IL-1β, TXNIP and TRX-1 were measured by Western blot analysis. After pretreatment of brusatol (300 nM) for 1 h, cells were exposed to Ori (10 μM) for 1 h and then treated with or without LPS (1 μg/ml) for 1 h or 24 h. a and e Protein expressions of Nrf2, HO-1, INOS, HMGB-1, P- IκBα and IκBα were measured by Western blot analysis. After pretreatment of TAK242 (5 μM) for 1 h, cells were exposed to Ori (10 μM) for 1 h and then treated with LPS (1 μg/ml) for 18 h. g Protein expressions of TLR4, P-P65, P65, P- IκBα and IκBα were measured by Western blot analysis. b, c, d, f and h Quantification of expressions of previous protein was performed by densitometric analysis, and β-actin acted as an internal control. All results were expressed as the means ± SEM of three independent experiments. *p < 0.05 and **p < 0.01 versus the control group, #p < 0.05 and ##p < 0.01 versus LPS group