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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Oridonin protects LPS-induced acute lung injury by modulating Nrf2-mediated oxidative stress and Nrf2-independent NLRP3 and NF-κB pathways

Fig. 3

Ori regulated Nrf2 protein expression via Akt and MAPK activation in RAW 264.7 cells. a, c, e and g Cells were pretreated with or without Akt inhibitor (20 μM), JNK inhibitor (20 μM), P38 inhibitor (10 μM) and ERK inhibitor (10 μM) for 18 h, and then exposed to Ori (10 μM) for an additional 6 h. b, d, f and h Quantification of induction of Akt and MAPK phosphorylation and total Nrf2 were performed by densitometric analysis and β-actin was acted as an internal control. All results were expressed as the means ± SEM of three independent experiments. *p <0.05 and **p < 0.01 versus the control group. +p < 0.05 and ++p < 0.01 versus Ori only group

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