Fig. 1

Ori inhibited cytotoxicity and ROS generation in RAW 264.7 cells. a The chemical structure of Oridonin (Ori). b Cells were exposed to Ori (2.5, 5 or 10 μM) for 1 h and then trea ted with or without hydrogen peroxide (300 μM) for another 18 h. Cell viability was determined by MTT assay. c Cells were treated with or without Ori for 18 h, stained with 50 μM of DCFH-DA for 30 min, and subsequently exposed to hydrogen peroxide (300 μM) for 10 min to induce ROS generation. DCF fluorescence intensities were detected by a fluorescence microscope. d and e According to the related instructions, LAL enzyme and LPS-LBP-binding assay were measured by the different absorbance. f and g Cells were exposed to Ori (2.5, 5 or 10 μM) for 1 h, treated with or without LPS (1 μg/ml) for another 18 h and stained with 50 μM of DCFH-DA for 30 min. DCF fluorescence intensities were detected by flow cytometry. All results were expressed as the means ± SEM of three independent experiments. *p < 0.05 and **p < 0.01 versus the control group, #p < 0.05 and ##p < 0.01 versus LPS group