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Table 2 Developmental function and regulation of Ids in different cell lineage in vitro

From: Inhibitor of DNA binding in heart development and cardiovascular diseases

Cell type

Treatment

Function

Regulation

Reference

P19CL6 cell

Id1/Id1 siRNA transfection

Promote/inhibit differentiation toward cardiomyocyte and cell proliferation

Up−/down- regulate Gata4, α-MHC, and Isl1

53

mESC

Id1 siRNA transfection

Inhibit cardiogenic mesoderm differentiation

Downregulate Kdr, Mesp1, Snai1, and Cdh11

45

mESC or hESC

Id1 transfection

Direct ESCs to differentiate toward the cardiogenic mesoderm

Upregulate Evx1, Grrp1, Mesp1, and Kdr, inhibit Tcf3 and Foxa2 expression

45

P19 cell

Id3 transfection

Inhibit cardiomyocyte differentiation

Inhibite the Expression of the Gata4, Nkx2.5, and MHC, such inhibition can be rescued by p204 protein

66

P19 cell

Crossveinless-2

Promote cardiomyocyte differentiation

Inhibit Smad1/5/8 activation and Id1 expression, enhance expression of T, Mesp1, Nkx2.5 and Tbx5

65

  1. α-MHC, α-myosin heavy chain; Cdh11, cadherin 11; Evx1, even-skipped homeobox 1; Foxa2, forkhead box A2; GATA4, GATA binding protein 4; Grrp1, glycine/arginine rich protein 1; Kdr, kinase insert domain receptor; Isl1, ISL LIM homeobox 1; m/hESC, mouse/human embryonic stem cell; Mesp1, mesoderm posterior bHLH transcription factor 1; Nkx2.5, NK2 homeobox 5; Smad1/5/8, SMAD family member 1/5/8; siRNA, small interfering RNA; Snai1, snail family transcriptional repressor 1; T, T-box transcription factor T or brachyury; Tbx5, T-box transcription factor 5; Tcf3, transcription factor 3