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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Novel mutant mouse line emphasizes the importance of protein kinase C theta for CD4+ T lymphocyte activation

Fig. 3

Similar changes in the T cell compartment of PKCθ-deficient and PKCθ-E2mut mice. a Total thymocyte counts were determined ex vivo using a Luna cell counter. The frequency of thymocyte subsets (DN, DP, CD4+ and CD8+ SP as well as nTregs) was analyzed by flow cytometry. Graphs summarizing all experiments are shown (the gating strategy and representative FACS dot blots are shown in Additional file 1: Figure S1). Each symbol represents values obtained of an individual mouse (n ≥ 5), the solid line shows the mean value. DN = Double negative; DP = double positive; SP = single positive. b Cell counts of combined spleen/lymph nodes was determined by Luna cell counter and the frequency of T cell subsets was analyzed by flow cytometry (the gating strategy and representative FACS dot blots are shown in Additional file 2: Figure S2). In (a) and (b), results of at least 4 independent experiments with a total of 5 or more mice per genotype are shown. Statistical analyses were performed using one-way ANOVA and Bonferroni’s multiple comparison test

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