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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Activin a promotes myofibroblast differentiation of endometrial mesenchymal stem cells via STAT3-dependent Smad/CTGF pathway

Fig. 6

Inhibition of Activin A pathway impeded myofibroblast differentiation of endometrial MSCs. Eutopic endometrial MSCs derived from patients without endometriosis (n = 3) were treated with 10 μM SB431542, 0.18 μg/mL αActivin or 0.18 μg/mL Follistatin for 1 h and then treated with 25 ng/mL recombinant human Activin A. After treatment with Activin A for 6 h, the expression of CTGF, α-SMA, collagen I, fibronectin was analyzed by Western blot (a). After treatment with Activin A for 1 h, p-Smad2, p-Smad3 and Smad2/3was analyzed by Western blot (b). The localization of Smad2/3 was examined by immunofluorescence analysis. The nuclei were stained with DAPI (c). The expression of Smad2/3 in the nuclear extract was analyzed by Western blot (d)

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