Skip to main content

Advertisement

Fig. 4 | Cell Communication and Signaling

Fig. 4

From: A piggyBac-based TANGO GFP assay for high throughput screening of GPCR ligands in live cells

Fig. 4

Use of the piggyBac-TANGO assay to profile a DRD2 agonist and an antagonist. We preincubated the DRD2-expressing HPT cells with their potential antagonist chlorpromazine. After 6 h, cabergoline was added into medium for additional 24 h exposure. a Fluorescence response of DRD2 induced by cabergoline and chlorpromazine in the piggyBac-TANGO assay. b Concentration-response curves of DRD2 stimulated by cabergoline and chlorpromazine in the piggyBac-TANGO assay. Data are presented as the means ± SEM (n = 3). Curves were fitted using GraphPad Prism 6

Back to article page