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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: A piggyBac-based TANGO GFP assay for high throughput screening of GPCR ligands in live cells

Fig. 3

Dose-response curves for the activation of the GPCRs. a-e Validation of selected receptors (GPR119, ADRB2, ADRB3, CB1 and DRD1) in the piggyBac-TANGO assay. The agonists of receptors were receptively MBX2982, isoproterenol (for ADRB2 and ADRB3), AM1202(CB1) and cabergoline. f-j Validation of selected receptors (DRD3, DRD5, MC4R, NUMR1 and NUMR2) in the piggyBac-TANGO assay. The agonists of receptors were receptively cabergoline (for DRD3 and DRD5), α-MSH(MC4R) and Neuromedin U-25 (for NUMR1 and NUMR2). Data are presented as the means ± SEM (n = 3). Curves were fitted using GraphPad Prism 6

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