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Fig. 8 | Cell Communication and Signaling

Fig. 8

From: Response to IL-6 trans- and IL-6 classic signalling is determined by the ratio of the IL-6 receptor α to gp130 expression: fusing experimental insights and dynamic modelling

Fig. 8

Pharmacological inhibition of Jak does not discriminate between trans- and classic signalling. a Ba/F3-gp130-IL-6Rα cells were pretreated with or without the indicated inhibitors (5 μM) for 30 min and subsequently stimulated with 10 ng/ml IL-6 (0.42 nM) or Hy-IL-6 (0.17 nM). STAT3 phosphorylation and STAT3 expression were evaluated by Western blotting. STAT3 expression served as loading control. Representative results of n = 3 independent experiments are shown. b Ba/F3-gp130-IL-6Rα cells were incubated with IL-6 (0.42 pM) or Hy-IL-6 (0.52 pM) with the indicated concentrations of the corresponding inhibitors for 48 h. Cell growth was measured using Cell Titer Blue reagent. Diamonds correspond to the mean and bars to the STD of n = 3 experiments. Red and blue lines indicate a maximum likelihood 4 parameter logistic regression. The determined IC50 values are given as dark stars for classic signalling and light grey stars for trans-signalling

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