Fig. 5

MIIP’s effect on AKT phoshphorylation and cell proliferation relies on interaction with PP1α. (a, b) C4–2-MIIP and PC3-MIIP cells were transfected with siNC, siPP1α#1 and siPP1α#2. Western blot analysis of p-AKT (Ser473) and p-AKT (Thr308) (a) and CCK8 assays analysis of cell viability (b) were performed. (c, d) C4–2 and PC3 cells were transiently transfected with pFLAG-CMV4-MIIP∆C or empty vector and 48 h later, subjected to Western blot and CCK8 assays. Data were means ± SEM. ^*p < 0.05