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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: MIIP inhibits the growth of prostate cancer via interaction with PP1α and negative modulation of AKT signaling

Fig. 4

MIIP interacts with Serine/Theonine Protein Phosphatase PP1α via its C-terminal part. (a) Western blot analysis of PP1α in stable control and MIIP-overexpressing LNCaP and PC3 cells. (b) Co-localization analysis MIIP and PP1α in HeLa cells co-transfected with pEGFP-N3-MIIP and pmCherry-C1-PP1α and in MIIP-HA-LNCaP cells transfected with pmCherry-C1-PP1α. (c) Co-immunoprecipitation analysis of MIIP and PP1α in 293 T cells co-transfected with pLEX-HA-MIIP and pmCherry-C1-PP1α, and in LNCaP-HA-MIIP cells transfected with pmCherry-C1-PP1α, by immunoprecipitation with anti-HA and immunoblot with anti-PP1α. (d) 293 T cells were co-transfected with pmCherry-C1-PP1α and pFLAG-CMV4-MIIP∆C or pFLAG-CMV4-MIIP∆N, and co-immunoprecipitation analysis were performed by immunoprecipitation with anti-FLAG and immunoblot with anti-PP1α

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Cell Communication and Signaling

ISSN: 1478-811X