Fig. 2

MIIP does not affect AR transcription activity and nuclear translocation. (a, b) Real-time RT-PCR analysis of AR and its target genes (PSA,NKX3.1, PMEPA1, SLC45A3, TMPRSS2, FKBP5) in stable control and MIIP-overexpressing LNCaP and C4–2 cells (A) and in LNCaP and 22Rv1 cells transfected with siNC or two individual MIIP-targeting siRNAs. (c) Real-time RT-PCR analysis of PSA and TMPRSS2 in stable control and MIIP-overexpressing LNCaP cells treated with Dihydrotestosterone (0, 50 nM,100 nM, 1 μM and 2 μM). (d) Real-time RT-PCR analysis of PSA in stable control and MIIP-overexpressing LNCaP and C4–2 cells treated with Enzalutamide (0, 10, 25, 50,100 and 200 nM). (e) Western blot analysis of cytosolic and nuclear AR protein in stable control and MIIP-overexpressing LNCaP cells treated with Enzalutamide (0, 25, 100). Data were means ± SEM in a-d