Fig. 1From: MIIP inhibits the growth of prostate cancer via interaction with PP1α and negative modulation of AKT signalingMIIP inhibits PCa cell proliferation. (a) Western blot analysis of MIIP protein expression in stable control and MIIP-overexpressing LNCaP and C4–2 cell lines. (b) Real-time RT-PCR analysis of MIIP mRNA level in stable cell lines. (c) The cell viability was determined by CCK8 assays at indicated time points. (d) Relative colony formation of stable cell lines. (e, f) LNCaP and 22Rv1 cells were transfected with siNC or two individual MIIP-targeting siRNAs. MIIP protein expression was examined by Western blot analysis (e) and the cell viability was determined by CCK8 assays (f). Data represent means ± SEM.*p < 0.05Back to article page