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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: ATG5 and ATG7 induced autophagy interplays with UPR via PERK signaling

Fig. 2

Expression of ER stress associated protein and autophagy related protein in the C28I2 chondrocyte infected with Ad-ATG5 or Ad-ATG7. a The ATG5 and ATG7 mRNA level were analysed by RT-PCR in C28I2 cells. b Analysis of ATG5 and ATG7 mRNA level with Q-PCR in C28I2 cells. Ad-ATG5(a), Ad-ATG7(b). c The ATG5 protein expression was analysed by western blotting in the Ad-ATG5 infected C28I2 cells. The level of ATG5 was normalized to β-actin. d The ATG7 protein expression was analysed by western blotting in the Ad-ATG7 infected C28I2 cells. The level of ATG7 was normalized to β-actin. e The expression levels of LC3, P62, PERK, p-PERK, Nrf2 proteins after infected with Ad-ATG5 in C28I2 cells for 24 h by western blotting. The levels of related proteins were normalized to β-actin. f The expression levels of LC3, P62, PERK, p-PERK, Nrf2 proteins after infected with Ad-ATG7 in C28I2 cells for 24 h by western blotting. The levels of related proteins were normalized to β-actin. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the controls. Values are means±SD n = 3). (1:NC, 2: Ad-GFP, 3:Ad-ATG5(a) and Ad-ATG7(b), 4:NC, 5:Ad-GFP, 6:Ad-ATG5, 7:Ad-ATG7)

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