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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Genomic and non-genomic pathways are both crucial for peak induction of neurite outgrowth by retinoids

Fig. 5

Neurite outgrowth of SH-SY5Y cells treated with retinoids. SH-SY5Y cells were treated with the retinoids or DMSO CT treatment for 5 days, and then neuronal differentiation was evaluated by immunofluorescence and morphometric analysis, using βIII-tubulin antibody. The average length of neurites extending from SH-SY5Y cells after a) 10 μM and b) 10 nM compound treatment was measured and compared with the control and ATRA. Shown are mean values of three independent experiments. The average neurite length was calculated by dividing the total neurite length by the total number of neurites in each micrograph. Error bars indicate SEM (* p < 0.05; *** p < 0.001; **** p < 0.0001 one-way ANOVA with Newman-Keuls multiple comparison test). c) Representative micrographs of SH-SY5Y cells stained with βIII-tubulin antibody after 10 nM retinoid treatment for 5 days (scale bar = 18 μm)

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Cell Communication and Signaling

ISSN: 1478-811X