Fig. 2

Cav-1 regulates activity of the FST promoter. Cav-1 WT and KO MC were transfected with (a) the full-length FST promoter luciferase construct or (b) the full-length FST promoter luciferase construct lacking intron 1 and exon 1. KO MC exhibited significantly elevated transcriptional activity of both constructs (n = 6, *p < 0.05). c Graphical representation of the synthesized FST promoter deletion constructs. d Cav-1 WT and KO MC were transfected with the FST promoter deletion constructs shown in (C). Transcriptional activation of all constructs was elevated in cav-1 KO MC (n = 6–20, *p < 0.05 vs WT for each construct). e, f Cav-1 knockdown in WT MC significantly increased the transcriptional activation of the -123 bp FST promoter (e) and FST protein expression (f) compared with control siRNA-transfected KO MC (for both, n = 6, *vs KO, #vs WT con siRNA, p < 0.05)