Fig. 8

Increased number of virions trapped in the endosomal hub in cells treated with Baf A1, but not NH₄Cl. Confocal analysis of ZIKV entry to Vero cells in the presence of intracellular agents hampering endosome acidification. Following 1 h pre-treatment with 100 nM Baf A1 or 50 mM NH₄Cl the cells were infected with ZIKV and fixed 40 min p.i. Baf A1 - ZIKV-infected Baf A1-treated cells; NH₄Cl - ZIKV-infected NH₄Cl -treated cells; vØ - ZIKV-infected inhibitor-untreated cells; mock – mock-infected, inhibitor-untreated cells. ZIKV envelope protein is visualized in green, actin cytoskeleton stained in red to show the cell boundaries and nuclei are shown in blue. Scale bar = 10 μm. Upper panel – xy projections; bottom panel – xz projections