Fig. 7
Baf A1 and NH4Cl inhibit ZIKV infection. Vero cells pre-treated with intracellular agents hampering endosome acidification were infected with ZIKV and viral yield was assessed at 3 days p.i. RT-qPCR results are presented on the left side of the graph. Right side of the graph shows viability of cells, as determined by an XTT assay. Mock – mock infected cells; vØ – ZIKV- infected cells; NH4Cl – 50 mM NH4Cl; BafA1–100 nM bafilomycin A1; control – inhibitor-untreated, non-infected cells. The data is presented as mean ± SD. To determine the significance of differences between compared groups, single-factor analysis of variance (ANOVA) was applied. P values < 0.05 were considered significant. One asterisk (*) identifies adjusted P values between 0.01 and 0.05, two asterisks (**) identify adjusted P values between 0.01 and 0.001, three asterisks (***) identify adjusted P values between 0.001 and 0.0001